A solution of lysozyme can be prepared in DNA-free water at 10 mg/mL. See the link below for further information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/458/sae0152pis.pdf
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SAE0152
Lysozym aus Hühnereiweiss
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥39,000 units/mg protein
Synonym(e):
Mucopeptid-N-Acetylmuramoylhydrolase, Muramidase
zur Ansicht der Organisations- und Vertragspreise.
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 g | Warenkorb auf Verfügbarkeit prüfen | € 78,20 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.77
MDL number:
€ 78,20
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Technischer Dienst
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Unterstützung erhaltenbiological source
chicken egg white
Quality Segment
form
powder
specific activity
≥39000 units/mg protein
mol wt
single-chain 14.3 kDa
feature
DNA free
technique(s)
cell based assay: suitable
input
bacteria and yeast null
suitability
suitable for cell lysis
application(s)
cell analysis
shipped in
ambient
storage temp.
−20°C
General description
Purified Lysozyme DNA free SAE0152 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥39,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥39,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Application
Enzym bricht die Zellwände von Bakterien, wird verwendet um Sphäroplasten vorzubereiten.
- Lysozyme is used for the extraction of genomic DNA from bacterial cells.
- Lysozyme is used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
- Lysonzyme is used to prepare spheroplasts.
Biochem/physiol Actions
Lysozym hydrolysiert β(1→4)-Bindungen zwischen N-Acetylmuraminsäure und N-Acetyl-D -Glucosamin-Resten in Peptidoglycan und zwischen N-Acetyl-D -Glucosamin-Resten in Chitodextrin. Gram-positive Zellen sind sehr anfällig für diese Hydrolyse, da ihre Zellwände einen hohen Peptidoglycan-Anteil haben.
Lysozym hydrolysiert β(1→4)-Bindungen zwischen N-Acetylmuraminsäure und N-Acetyl-D -Glucosamin-Resten in Peptidoglycan und zwischen N-Acetyl-D -Glucosamin-Resten in Chitodextrin. Gram-positive Zellen sind sehr anfällig für diese Hydrolyse, da ihre Zellwände einen hohen Peptidoglycan-Anteil haben. Gram-negative Bakterien sind auf Grund einer äußeren Membran und eines geringeren Peptidoglycan-Anteils weniger anfällig. Allerdings können diese Zellen in Gegenwart von EDTA, welches Chelate mit Metallionen in der äußeren bakteriellen Membran bildet, hydrolysiert werden.
Das Enzym ist über einen breiten pH-Bereich aktiv (6.0 bis 9.0). Bei pH 6.2 wird eine maximale Aktivität über einen größeren Ionenstärken-Bereich (0.02 bis 0.100 M), verglichen mit pH 9,2 (0.01 bis 0.06 M), beobachtet.
Das Enzym ist über einen breiten pH-Bereich aktiv (6.0 bis 9.0). Bei pH 6.2 wird eine maximale Aktivität über einen größeren Ionenstärken-Bereich (0.02 bis 0.100 M), verglichen mit pH 9,2 (0.01 bis 0.06 M), beobachtet.
Features and Benefits
Purified Lysozyme free SAE0152 undergoes strict quality control testing to ensure it will be Free of DNA contaminants, suitable for Microbiome research.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
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Dieser Artikel | |||
|---|---|---|---|
| form powder | form lyophilized powder | form lyophilized solid | form powder or granules |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. room temp |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level 200 |
| specific activity ≥39000 units/mg protein | specific activity ≥40,000 units/mg protein | specific activity ≥20,000 Shugar units/mg dry wt | specific activity ≥39,000 units/mg protein |
| suitability suitable for cell lysis | suitability suitable for cell lysis | suitability suitable for cell lysis | suitability suitable for cell lysis |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hello. I need to prepare 50mg/ml solution. What is the best solvent/buffer to use? Thank you.
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