Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

R1508

Sigma-Aldrich

Pvu I from Proteus vulgaris

Restriction Enzyme

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

CAS-Nummer:
81295-33-2
EC-Nummer:
3.1.21.4 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00166579
UNSPSC-Code:
12352204

Qualität

for molecular biology

Form

buffered aqueous glycerol solution

Konzentration

≥5000 units/mL
5,000 units/mL

Versandbedingung

wet ice

Lagertemp.

−20°C

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Spezifität

Recognition sequence: 5′-CGAT/CG-3′
Cutting results: A 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Anwendung

PvuI is a restriction endonuclease that is used for molecular biological applications to cleave DNA at the recognition sequence 5′-CGAT/CG-3′ to generate fragments with 3′-cohesive termini.

Sonstige Hinweise

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Physikalische Form

Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 300 mM KCl, 1 mM dithioerythritol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C.

Ähnliches Produkt

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

T R Gingeras et al.
Nucleic acids research, 9(18), 4525-4536 (1981-09-25)
Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5'
Sanbing Shen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(43), 10893-10904 (2008-10-24)
Disrupted-in-Schizophrenia-1 (DISC1), identified by positional cloning of a balanced translocation (1;11) with the breakpoint in intron 8 of a large Scottish pedigree, is associated with a range of neuropsychiatric disorders including schizophrenia. To model this mutation in mice, we have
E Bonnelye et al.
The Journal of cell biology, 153(5), 971-984 (2001-05-31)
The orphan nuclear estrogen receptor-related receptor alpha (ERRalpha), is expressed by many cell types, but is very highly expressed by osteoblastic cells in which it transactivates at least one osteoblast-associated gene, osteopontin. To study the putative involvement of ERRalpha in
TALENs and ZFNs are associated with different mutation signatures.
Yongsub Kim et al.
Nature methods, 10(3), 185-185 (2013-02-12)
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.