P5899

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Synonym(e): PDL HBr
• CAS-Nummer: 25988-63-0
• UNSPSC Code: 12352202
• eCl@ss: 32160406
• PubChem Substance ID: 24898718
• NACRES: NA.75
• MDL number: MFCD00237230
• Biological source: synthetic (organic)
• Form: lyophilized powder
• Technique(s): cell culture | mammalian: suitable
• Mol wt: ≥300,000

Eigenschaften

• Produktname: Poly-L-Lysin -hydrobromid, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChI key: WBSCNDJQPKSPII-KKUMJFAQSA-N
• biological source: synthetic (organic)
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• mol wt: ≥300,000
• packaging: pkg of 5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 4 μg/cm²
• impurities: <10% water (by Karl Fischer)
• solubility: H₂O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow
• shipped in: ambient
• storage temp.: −20°C
• Quality Level: 200

Beschreibung

Application

Poly-L-lysine hydrobromide has been used to coat:

• 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation[1]
• flat bottom 96-well plates for in vitro neuronal stem cell differentiation[2]
• T75 flasks for primary astrocytic cultures[3]

Biochem/physiol Actions

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

Poly-L-Lysin ist ein positiv geladenes Aminosäurepolymer mit etwa einem HBr pro Lysin-Rest. Das Hydrobromid ermöglicht die kristalline, wasserlösliche Form des Poly-L-Lysins. Eine geringe Produktmenge kann in der β-Struktur zu finden sein, da das HBr die Wasserstoffbindung zwischen Aminogruppen und den Carboxylgruppen oder N oder O enthaltenden Gruppen beeinträchtigt.

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Disclaimer

Sterile Lösungen sind bei Lagerung zwischen 2 und 8 °C bis zu 2 Jahre haltbar. Das Lyophilisat sollte bei -20 °C aufbewahrt werden.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)