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Merck

P5368

Sigma-Aldrich

Phosphatgepufferte Kochsalzlösung -Lösung

powder, pH 7.4, BioPerformance Certified, for molecular biology

Synonym(e):

PBS

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About This Item

MDL-Nummer:
UNSPSC-Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphatgepufferte Kochsalzlösung -Lösung, BioPerformance Certified, pH 7.4

Qualität

BioPerformance Certified
for molecular biology

Form

powder

Lagerbedingungen

(Keep container tightly closed in a dry and well-ventilated place.)

Methode(n)

immunofluorescence: suitable

Verunreinigungen

BioBurden, tested
endotoxin, tested

Farbe

white

pH-Wert

7.4

Löslichkeit

water: 9.07-10.0 g/L, clear, colorless

Kationenspuren

Fe: ≤10 ppm
heavy metals (as Pb): ≤5 ppm

Eignung

suitable for SDS-PAGE
suitable for molecular biology

Anwendung(en)

diagnostic assay manufacturing
histology
sample preparation

Fremdaktivität

DNase, RNase, Protease, none detected

SMILES String

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChIKey

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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Anwendung


  • Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: This study used phosphate buffered saline (PBS) to develop a cost-effective and efficient method for DNA extraction from insects, facilitating routine PCR-based applications in molecular biology (Jangra & Ghosh, 2022).

  • Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS was utilized to prepare and deliver adipose-derived stem cells in an injectable hydrogel, showing potential for innovative osteoarthritis treatments (Bhattacharjee et al., 2022).

  • Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: This research employed PBS for sample preparation in a novel LC-MS method for detecting SARS-CoV-2, providing a rapid and sensitive diagnostic tool (Hober et al., 2021).

Verpackung

Folienbeutel

Rekonstituierung

Der Inhalt eines Beutels, in einem Liter destilliertem oder entionisiertem Wasser gelöst, ergibt eine 0,01 M phosphatgepufferte Kochsalzlösung (NaCl – 0,138 M; KCl – 0,0027 M), pH 7,4 bei 25 °C.

Sonstige Hinweise

Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Jesús Gilberto Arámburo-Galvez et al.
Nutrients, 10(7) (2018-07-18)
Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing
Petra J Roubos-van den Hil et al.
Veterinary microbiology, 202, 58-63 (2016-09-27)
Enterotoxigenic E. coli (ETEC), causing post-weaning diarrhoea, is a major problem in weaned piglets. Individual animal responses to ETEC infection show high variability in animal experiments. Two studies were designed to optimize the ETEC F4ac infection model in piglets by
Jinjin Sun et al.
Nanoscale research letters, 14(1), 248-248 (2019-07-26)
Herein, a magnetic graphene field-effect transistor biosensor was prepared through the transfer of a chemical vapor deposition graphene film onto a glass substrate to produce a sensing film and conductive channel. By fixing 1-pyrenebutanoic acid succinimidyl ester onto graphene film
Gary Burness et al.
Biology open, 7(4) (2018-04-06)
Female birds transfer maternally derived antibodies (matAb) to their nestlings, via the egg yolk. These antibodies are thought to provide passive protection, and allow nestlings to avoid the costs associated with mounting an innate immune response. To test whether there
Yixi Chen et al.
The European journal of neuroscience, 49(4), 510-524 (2018-11-26)
An emerging treatment for Parkinson's disease (PD) is cell replacement therapy. Authentic midbrain dopaminergic (mDA) neuronal precursors can be differentiated from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). These laboratory-generated mDA cells have been demonstrated

Artikel

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The application note describes a comparative analysis of plasma protein binding results for free/bound drugs using two different sample extraction steps, with and without agitation.

Learn about Supel™ BioSPME C18 high-throughput devices to determine plasma protein binding and decrease the sample preparation time to less than an hour.

Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.

Protokolle

Preparation for biodegradable nanoparticles and their use in transfection protocols .

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