O4878

Oxalacetat-Decarboxylase aus Pseudomonas sp.

lyophilized powder, ≥100 units/mg solid

• Synonym(e): OAD
• CAS-Nummer: 9024-98-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 4.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131775
• Specific activity: ≥100 units/mg solid

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥100 units/mg solid
• storage temp.: −20°C

Beschreibung

General description

Oxaloacetate decarboxylase or OAD functions as a Na pump in anaerobic bacteria. It is a membrane protein consisting of three subunits, α, β and γ with the α subunit containing the carboxylase activity.

Application

Oxaloacetate Decarboxylase from Pseudomonas sp. has been used in the digestion of the low molecular weight (LMW) human milk fraction (5kF fraction) and as a positive control for deciphering C. thermocellum oxaloacetate decarboxylase activity.

Oxaloacetate decarboxylase has been used in a study to assess turnover and accessibility of a reentrant loop of the Na(+)-glutamate transporter GltS. It has also been used in a study to investigate fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources.

Biochem/physiol Actions

Oxaloacetate Decarboxylase catalyzes the decarboxylation of oxaloacetate and requires manganese and magnesium for its activity. It is associated with a wide vareity of Gram-negative bacteria.

Other Notes

1 U setzt 1.0 μmol Oxalacetat zu Pyruvat und CO₂ pro Minute um bei pH 8.0 und 25°C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)