O2260

Mouse Osteopontin

recombinant, expressed in NSO cells, powder, suitable for cell culture

• Synonym(e): Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• MDL-Nummer: MFCD03100698
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• product name: Osteopontin from mouse, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Rekombinant: expressed in NSO cells
• Sterilität: sterile
• Assay: >95% (SDS-PAGE)
• Form: lyophilized powder
• Mol-Gew.: 30 kDa by SDS-PAGE (reducing); 65 kDa by SDS-PAGE (reducing) (doublet)
• Verpackung: pkg of 50 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P10923
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Spp1(20750)

Beschreibung

Anwendung

Osteopontin from mouse has been used:

• to study its role in a mouse model of preterm brain injury
• as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
• to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels

Biochem./physiol. Wirkung

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Hinweis zur Analyse

The biological activity is measured by its ability to mediate 293 cell adhesion.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable