One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.
Fortfahren mit
N7271
α(2→3)-Neuraminidase aus Streptococcus pneumoniae
buffered aqueous solution
Synonym(e):
Acyl-Neuraminyl-Hydrolase, Acylneuraminyl-Hydrolase, Receptor-destroying enzyme, Sialidase
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
0.2 unit | Warenkorb auf Verfügbarkeit prüfen | € 1.110,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Biological source:
Streptococcus pneumoniae
Concentration:
≥5 units/mL
€ 1.110,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenbiological source
Streptococcus pneumoniae
Quality Segment
form
buffered aqueous solution
concentration
≥5 units/mL
foreign activity
β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Gene Information
Streptococcus pneumoniae R6 ... nanB(933504)
Biochem/physiol Actions
Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Physical form
Solution in 50 mM sodium phosphate, pH 7.5
Preparation Note
Expressed in glycosidase-free hosts.
Other Notes
One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| Gene Information Streptococcus pneumoniae R6 ... nanB(933504) | Gene Information Clostridium perfringens str. 13 ... nanI(988807) | Gene Information Clostridium perfringens str. 13 ... nanI(988807) | Gene Information Clostridium perfringens str. 13 ... nanI(988807) |
| biological source Streptococcus pneumoniae | biological source - | biological source - | biological source Clostridium perfringens str. 13 |
| concentration ≥5 units/mL | concentration - | concentration - | concentration - |
| form buffered aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.
Helpful?
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What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.
Helpful?
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For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?
1 answer-
The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.
Helpful?
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