One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.
N7271
α(2→3)-Neuraminidase aus Streptococcus pneumoniae
buffered aqueous solution
Synonym(e):
Acyl-Neuraminyl-Hydrolase, Acylneuraminyl-Hydrolase, Receptor-destroying enzyme, Sialidase
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
0.2 UNIT
€ 1.110,00
€ 1.110,00
Warenkorb auf Verfügbarkeit prüfen
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Biological source:
Streptococcus pneumoniae
Concentration:
≥5 units/mL
Fortfahren mit
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
Streptococcus pneumoniae
form
buffered aqueous solution
concentration
≥5 units/mL
foreign activity
β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
Streptococcus pneumoniae R6 ... nanB(933504)
Verwandte Kategorien
1 of 4
Dieser Artikel | N8271 | N6514 | N7885 |
|---|---|---|---|
| biological source Streptococcus pneumoniae | biological source - | biological source Vibrio cholerae | biological source - |
| Gene Information Streptococcus pneumoniae R6 ... nanB(933504) | Gene Information - | Gene Information - | Gene Information - |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| concentration ≥5 units/mL | concentration - | concentration - | concentration - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Biochem/physiol Actions
Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Physical form
Solution in 50 mM sodium phosphate, pH 7.5
Preparation Note
Expressed in glycosidase-free hosts.
Other Notes
One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Dominic Meusch et al.
Nature, 508(7494), 61-65 (2014-02-28)
Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures
Johan Nordholm et al.
The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
Audu J Natala et al.
Journal of medical entomology, 50(1), 85-93 (2013-02-23)
Amblyomma variegatum F. are obligate hematophagous ectoparasites of livestock that serve as the vectors of Ehrlichia ruminantium (formerly known as Cowdria ruminantium), the causative agent of heartwater disease. In the light of the fact that they are blood-feeding, their salivary
Patricia J Campbell et al.
Journal of virology, 88(7), 3802-3814 (2014-01-17)
The 2009 H1N1 lineage represented the first detection of a novel, highly transmissible influenza A virus genotype: six gene segments originated from the North American triple-reassortant swine lineage, and two segments, NA and M, derived from the Eurasian avian-like swine
Tadatsugu Imamura et al.
Journal of virology, 88(5), 2374-2384 (2013-12-29)
Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying
Artikel
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
Verwandter Inhalt
Datasheet
-
What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
Helpful?
-
-
What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?
1 answer-
The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.
Helpful?
-
-
What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung