The supplier advises that they have not yet validated any lysis buffers for use with cell samples in conjunction with this kit. Additionally, they recommend using an untreated, clear, flat-bottom plate for colorimetric measurements. Therefore, it would be preferable to transfer the samples to such a plate for running the assay.
Fortfahren mit
MAK317
Sorbitol Dehydrogenase Assay Kit
sufficient for 100 colorimetric tests
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 441,00 |
Über diesen Artikel
NACRES:
NA.84
UNSPSC Code:
12161503
Technischer Dienst
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Unterstützung erhaltendetection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; gastrointestinal diseases
storage temp.
−20°C
General description
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.
Application
Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.
Biochem/physiol Actions
Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).
Features and Benefits
Compatible with high-throughput handling systems.
Disclaimer
Supply conditions do not apply to the regions and states of Brazil: North, Northeast, Mato Grosso do Sul, Mato Grosso, and Rio Grande do Sul.
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Dieser Artikel | |||
|---|---|---|---|
| description sufficient for 100 colorimetric tests | description Sufficient for 100 colorimetric tests | description sufficient for 100 fluorometric tests | description Sufficient for 100 colorimetric tests |
| detection method colorimetric | detection method colorimetric | detection method fluorometric | detection method colorimetric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) endocrinological disorders, diabetes; gastrointestinal diseases | relevant disease(s) endocrinological disorders, diabetes; hematological disorder | relevant disease(s) cardiovascular diseases | relevant disease(s) endocrinological disorders, diabetes; genitourinary disorders |
Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklasse
3 - Flammable liquids
Flammpunkt (°F)
75.2 °F - closed cup
Flammpunkt (°C)
24 °C - closed cup
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What are the recommended methods for homogenizing or lysing cells in a 96-well plate for the MAK317 Sorbitol Dehydrogenase assay kit? The manual advises against using proteolytic enzymes and suggests using a rubber policeman, which is not suitable for a 96-well plate. Are there any recommendations for a mild lysing buffer with minimal impact on the assay outcome? Additionally, is it possible and recommended to run the assay in the same 96-well plate, or is transferring the samples to a new plate necessary?
1 answer-
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