Samples from murine blood that have detectable concentrations of renin should be suitable for use with the assay.
Fortfahren mit
MAK157
Renin Assay Kit
sufficient for 100 fluorometric tests
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(e):
Renin Enzyme Detection Kit
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 320,00 |
Über diesen Artikel
NACRES:
NA.84
UNSPSC Code:
12161503
Technischer Dienst
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Unterstützung erhaltenusage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cardiovascular diseases
storage temp.
−20°C
Gene Information
human ... REN(5972)
rat ... REN(24715)
General description
Renin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension.
Application
Renin Assay Kit has been used to determine the activity of renin.
The Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors.
Biochem/physiol Actions
Renin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (λex = 540/λem = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay.
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Dieser Artikel | |||
|---|---|---|---|
| description sufficient for 100 fluorometric tests | description for serum, plasma and cell culture supernatants | description for serum, plasma and cell culture supernatants | description sufficient for 100 colorimetric or fluorometric tests |
| Gene Information human ... REN(5972) | Gene Information mouse ... Ren1(19701) | Gene Information human ... REN(5972) | Gene Information - |
| detection method fluorometric | detection method colorimetric | detection method colorimetric | detection method fluorometric, colorimetric |
| usage sufficient for 100 fluorometric tests | usage - | usage - | usage sufficient for 100 colorimetric or fluorometric tests |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) cardiovascular diseases | relevant disease(s) - | relevant disease(s) - | relevant disease(s) cardiovascular diseases; immunological diseases |
Lagerklasse
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can the Renin assay kit MAK157 be used on murine blood samples?
1 answer-
Helpful?
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What is the molar concentration of the renin substrate (1 ug/mL)?
1 answer-
The concentration of the 100x renin substrate solution is 2 mM.
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Species that this assay works on?
1 answer-
Although the standard that has been tested is recombinant human renin, theoretically the kit should be suitable for all other species.
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Does the kit MAK157 contain any Renin inhibitor? If so, how much Renin inhibitor should be added, and at what step of the assay should it be added in order to determine the Renin activity?
1 answer-
The kit MAK157 does not contain any Renin inhibitor. To confirm the activity using this kit, researchers can use a RENIN INHIBITOR separately.The amount of inhibitor to use and the step to use the Renin inhibitor can be referred to from published literature.
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