The standard in MAK121C is sialic acid. MAK121D enzymes catalyze reactions that convert sialic acid to pyruvate, which then reacts with a dye reagent to yield a quantifiable color signal. Heat-inactivation is not required or recommended. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/409/222/mak121pis-ms.pdf
Fortfahren mit
MAK121
Neuraminidase-Aktivitäts-Assay-Kit
sufficient for 100 colorimetric or fluorometric tests
Neuraminidase Activity Assay Kit
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(e):
Sialidase-Aktivitäts-Assay-Kit
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 690,00 |
Über diesen Artikel
NACRES:
NA.84
UNSPSC Code:
12161503
Technischer Dienst
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Unterstützung erhaltenusage
sufficient for 100 colorimetric or fluorometric tests
application(s)
pharmaceutical
detection method
colorimetric, fluorometric
shipped in
dry ice
storage temp.
−20°C
General description
Die auch als Sialidase bezeichnete Neuraminidase ist ein Enzym, das terminale Sialinsäurereste auf Polysacchariden-Ketten hydrolisiert. Sie wird hauptsächlich in Mikroorganismen wie Bakterien und Viren exprimiert. Es wird angenommen, dass die Spaltung von Sialinsäureresten durch Neuraminidase bei der Infektion durch Gruppeviren mehrere Rollen spielt. Weiterhin wird davon ausgegangen, dass sie die Penetration von Schleimhäuten, den Befall von Zielzellen, die Elution von Nachkommenviren aus infizierten Zellen und die Prävention einer Selbstaggregation unterstützt. Daher ist die Neuraminidase ein wichtiges Ziel für die Entwicklung von Influenza-Wirkstoffen und für einfache, direkte und automatisierungsbereite Verfahren für das Messen der Neuraminidase-Aktivität in breitgefächerten Anwendungen im Forschungsbereich und bei der Wirkstoffentwicklung
Application
- 5-Nitrobenzo[c][1, 2, 5]selenadiazole as therapeutic agents in the regulation of oxidative stress and inflammation induced by influenza A(H1N1)pdm09 in vitro and in vivo.: In dieser Forschungsarbeit wurde das Neuraminidase-Activity-Assay-Kit zur Untersuchung therapeutischer Wirkstoffe eingesetzt, die oxidativen Stress und Entzündungen regulieren. Dabei wurde seine entscheidende Rolle für die antivirale Forschung und potenzielle pharmakologischen Anwendungen aufgezeigt (Guo et al., 2023).
- Potency of bacterial sialidase Clostridium perfringens as antiviral of Newcastle disease infections using embryonated chicken egg in ovo model.: Erläutert die Verwendung des Kits zur Messung und Beobachtung der Sialidase-Enzymaktivität zur Beurteilung des antiviralen Potenzials bakterieller Enzyme gegen Geflügelkrankheiten und trägt so zur Entwicklung neuartiger Biokontrollmethoden beitragen (Kurnia et al., 2022).
- Molecular Docking Studies and Biological Evaluation of Berberine-Benzothiazole Derivatives as an Anti-Influenza Agent via Blocking of Neuraminidase.: Verwendet Neuraminidase-Assay-Kit zur Bewertung der Wirksamkeit der Neuraminidase-Aktivität der synthetisierten Verbindung und bietet einen Weg zur Entwicklung neuer antiviraler Medikamente mit verbesserter Spezifität und Wirksamkeit (Kumar et al., 2021).
Für den Nachweis der Neuraminidase-Aktivität in biologischen Proben und für das Screening von Neuraminidase-Inhibitoren geeignet.
Biochem/physiol Actions
In diesem Test wird die Neuraminidase-Aktivität mithilfe eines Enzym-Assays bestimmt, bei dem von der Neuraminidase freigesetzte Sialinsäure in einem kolometrischen (570 nm)/fluorometrischen (λex = 530/λem = 585 nm) Produkt resultiert, das zur Neuraminidase-Aktivität in der Probe direkt proportional ist. Der lineare Detektionsbereich bei 37 °C beträgt 0,1–10 Einheiten/L für kolorimetrische Assays und 0,01–2 Einheiten/L für fluorometrische Assays.
Features and Benefits
Kompatibel mit Handhabungssystemen mit hohem Durchsatz.
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Dieser Artikel | |||
|---|---|---|---|
| description sufficient for 100 colorimetric or fluorometric tests | description sufficient for 100 colorimetric or fluorometric tests | description Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose) | description sufficient for 100 tests (Ultraviolet) |
| detection method colorimetric, fluorometric | detection method colorimetric, fluorometric | detection method - | detection method colorimetric |
| usage sufficient for 100 colorimetric or fluorometric tests | usage sufficient for 100 colorimetric or fluorometric tests | usage - | usage sufficient for 100 tests (Ultraviolet) |
| shipped in dry ice | shipped in - | shipped in - | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| application(s) pharmaceutical | application(s) cosmetics | application(s) diagnostic assay manufacturing | application(s) - |
signalword
Danger
Lagerklasse
10 - Combustible liquids
wgk
WGK 2
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
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Is the standard provided (MAK121C) sialic acid? What is the catalytic function of the enzyme (MAK121D)? If the sample is serum, is it recommended to heat-inactivate it before the assay? Thank you.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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What is the pH of the assay buffer?
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The assay buffer is a pH of 7.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is it possible to use the Neuraminidase Activity Assay Kit (MAK121-1KT) for testing bacterial cultures, given that references indicate the use of plasma, serum, and purified enzymes as samples?
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The kit has been verified for use with bacterial cultures. Although there isn't a tailored sample preparation protocol for bacterial cultures, the following general cell lysate preparation method is suggested:
To prepare cell samples, first, suspend approximately two million harvested cells (2 × 10^6) in 400 μL of PBS on ice. Cell lysis can be accomplished by either using a Dounce homogenizer for 10-20 passes while on ice or through sonication in an ice-water bath. The effectiveness of lysis should be verified under a microscope. After lysis, centrifuge the homogenate at 14,000 g for 10 minutes and then transfer the clear supernatant to a clean tube. It is recommended to run a pilot assay with different sample dilutions to identify a dilution that falls within the linear range of the standard curve for accurate assays. If immediate analysis is not possible, most samples can be preserved at -80°C.Helpful?
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What type of sialic acid linkage is present in this kit? Specifically, is it an α2,3- or α2,6-linkage?
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The substrate utilized is fetuin, which is a heavily sialylated protein derived from fetal bovine serum. The specific type of sialic acid linkage present in this substrate has not been characterized. However, fetuin typically exhibits two types of linkages: α2,3- and α2,6-linkages. It is more common for α2,3-linkages to be present in fetuin substrates, which suggests that experiments targeting this linkage type are likely to be successful. Nonetheless, it is not possible to provide a definitive statement on the exact type of linkage present.
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Hallo, welches MQ Level hat das Material MAK121?
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These kits is considered an out of scope in terms of the MQ Classification system, but are handled and supported similarly to the MQ100 level products.
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