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Merck

MAK014

Sigma-Aldrich

Glucose-6-Phosphate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric tests

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

hematological disorder

Lagertemp.

−20°C

Allgemeine Beschreibung

Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.[1]

Anwendung

Glucose-6-phosphate assay kit has been used in:

  • glucose 6-phosphate (G6P) assay[2]
  • quantification of glucose-6-phosphate (G6P)[3][4][5] and fructose-6-phosphate (F6P)[3]
  • metabolite analysis[6]

Eignung

Suitable for quantifying Glucose-6-Phosphate in a variety of samples.

Prinzip

G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.

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Preisangaben

Piktogramme

Health hazardCorrosion

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials


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