M3942

DMEM - high glucose

MegaCell^™, with sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell cutlure

• Synonym(e): DMEM, DMEM Supplement
• UNSPSC-Code: 12352207
• NACRES: NA.75

Eigenschaften

• Produktbezeichnung: MegaCell^™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture
• Sterilität: sterile-filtered
• Form: liquid
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Komponenten: L-glutamine: no; glucose: 4500 mg/L (Dextro); sodium pyruvate: yes
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

MegaCell^™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Anwendung

MegaCell^™ Dulbecco′s Modified Eagle′s Medium has been used:

• as a component of the M10 medium to culture CD133⁺ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
• as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
• to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Rekonstituierung

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Rechtliche Hinweise

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.

MegaCell is a trademark of Promega Corp.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable