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M3770

Micrococcus lysodeikticus ATCC No. 4698

suitable for substrate for the assay of lysozyme, lyophilized cells

Synonym(e):

Micrococcus luetus

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M3770-5G

€ 162,00

M3770-25G

€ 638,00

M3770-100G

€ 1.000,00

€ 162,00

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Über diesen Artikel

NACRES:
NA.54
UNSPSC Code:
12352204

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form

lyophilized cells

Quality Level

200

suitability

suitable for substrate for the assay of lysozyme

storage temp.

−20°C

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Dieser Artikel
M0508SRE0018N3755
Micrococcus lysodeikticus ATCC No. 4698 suitable for substrate for the assay of lysozyme, lyophilized cells

Sigma-Aldrich

M3770

Micrococcus lysodeikticus ATCC No. 4698

Micrococcus lysodeikticus ATCC No. 4698 lyophilized cells

Sigma-Aldrich

M0508

Micrococcus lysodeikticus ATCC No. 4698

Lyticase from Arthrobacter Luteus Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein

Sigma-Aldrich

SRE0018

Lyticase from Arthrobacter Luteus

Nuclease micrococcal from Staphylococcus aureus 100-300 units/mg protein

Sigma-Aldrich

N3755

Nuclease micrococcal from Staphylococcus aureus

suitability

suitable for substrate for the assay of lysozyme

suitability

-

suitability

-

suitability

-

form

lyophilized cells

form

lyophilized cells

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

300

Application

Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. [1] It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles. [2]
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes. [3]

Biochem/physiol Actions

Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.

Analysis Note

Contains polynucleotide phosphorylase.

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number
0000522923
0000522923
0000480950
0000480950
0000480949

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Ghosh et al.
Biochemical engineering journal, 6(1), 19-24 (2000-07-26)
This paper discusses the purification of lysozyme from chicken egg white using hollow-fibre ultrafiltration (30kDa MWCO, polysulphone membrane). Lysozyme is preferentially transmitted through the membrane while the membrane largely retains other egg white proteins. Improvement in system hydrodynamics resulted in
Jingwei Xie et al.
Journal of colloid and interface science, 317(2), 469-476 (2007-10-20)
A co-axial electrospray process was developed to encapsulate protein-based drugs in biodegradable polymeric microparticles eliminating the key problem faced by other conventional methods of protein encapsulation--the primary emulsion being a major cause for protein denaturation and aggregation. Bovine serum albumin
Mathieu Giraudeau et al.
PloS one, 5(10), e13555-e13555 (2010-11-05)
As egg production and offspring care are costly, females should invest resources adaptively into their eggs to optimize current offspring quality and their own lifetime reproductive success. Parasite infections can influence maternal investment decisions due to their multiple negative physiological
Thais Heloisa Vaz Farias et al.
Fish & shellfish immunology, 101, 186-191 (2020-04-05)
Aeromonas hydrophila is responsible for outbreaks of a severe infectious disease in fish farms around the world and is one of the major causes of economic losses to the neotropical fish farmers. This study assessed the induction of immune responses
Ingrid Bourgeois et al.
FEMS microbiology letters, 290(1), 105-113 (2008-11-26)
The nucleotide sequence of atlL, a gene encoding a putative Staphylococcus lugdunensis peptidoglycan hydrolase, was determined using degenerate consensus PCR and genome walking. This 3837-bp gene encodes a protein, AtlL, that appears as a putative bifunctional autolysin with a 29-amino
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Protokolle

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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