L9267

Lectin from Lens culinaris (lentil)

lyophilized powder

• Synonym(e): Lens culinaris agglutinin, LcH
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized powder

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• potency: <16 μg per mL agglutination activity
• impurities: salt, free
• storage temp.: 2-8°C

Beschreibung

General description

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Application

Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).

Biochem/physiol Actions

LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.

Preparation Note

Highly purified by affinity chromatography.

Analysis Note

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)