This product is assigned retest date of approximately 3 years from the date of Quality Release. This date is lot-specific and may be found in the product Certificate of Analysis. When stored at room temperature, in a tightly sealed container, the material will be stable at least until the assigned retest date.
Fortfahren mit
L7275
LB Bouillon
Tablet microbial growth medium
Synonym(e):
Luria Bertani Bouillon
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 tabs | Warenkorb auf Verfügbarkeit prüfen | € 118,00 |
500 tabs | Warenkorb auf Verfügbarkeit prüfen | € 412,00 |
Über diesen Artikel
NACRES:
NA.85
UNSPSC Code:
12352200
€ 118,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Segment
sterility
non-sterile
form
tablet
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Die als Trockennährmedium erhältliche Luria-Bertani (LB)-Bouillon wird als Lennox LB bezeichnet und verfügt über Aminosäuren als Kohlenstoffquelle.
Lennox LB ist ein gerne genutztes Medium für das Mikrobenwachstum, das auch für die Kultivierung von E. coli verwendet wird. Die nährstoffreiche mikrobielle Bouillon enthält Peptide, Aminosäuren und Kohlenhydrate in einer Formulierung mit niedrigem Salzgehalt.
Application
Die LB-Bouillon (Lennox) wird für die Kultivierung von Escherichia coli und Bacterial Artificial Chromosomes (BACs) verwendet.
Geeignet für die nicht selektive Kultivierung von E. coli-Stämmen für das Klonen, die DNA-Plasmidproduktion und die Produktion rekombinanter Proteine. Ebenso geeignet für die selektive Kultivierung bei entsprechender Zugabe von Antibiotika, einschließlich derer, die Lösungen mit geringem Salzanteil erfordern, wie Zeocin®.
Features and Benefits
Eigenschaften der Lennox LB-Tabletten:
- Praktische, kleine Packung macht Wiegen unnötig
- Schnelle Präparation – kein Rühren erforderlich
Preparation Note
Je eine 1,1-g-Tablette in 48,3 ml Wasser geben (kein Rühren erforderlich). Zur Sterilisierung 20 Minuten lang bei 121 °C autoklavieren. Bei Bedarf vor der Zugabe weiterer Stoffe, z. B. Antibiotika, abkühlen lassen.
Other Notes
10 g/l Pepton
5 g/l Hefeextrakt
5 g/l NaCl
2,2 g/l inertes Bindemittel
5 g/l Hefeextrakt
5 g/l NaCl
2,2 g/l inertes Bindemittel
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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Dieser Artikel | |||
|---|---|---|---|
| description Tablet microbial growth medium | description Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture. | description EZMix powder microbial growth medium | description Powder microbial growth medium |
| suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for coliforms, nonselective for Escherichia coli, suitable for molecular biology |
| form tablet | form powder | form powder | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) food and beverages | application(s) agriculture | application(s) food and beverages |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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How long can these tablets be stored at room temperature in the bottle (after opening).
1 answer-
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Do the tablets contain any additives to adjust the pH of the media? Also, what should the final pH of the media be?
1 answer-
The pH of the media can be 7.0, but depending on the application, it can range from 7.0 to 8.0. The pH can be adjusted using 1N NaOH and 1N HCl.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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What is the difference among the many LB - Lennox products?
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LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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