This product has not been tested for stability once dissolved in water. It is recommended to reconstitute just before use.
Fortfahren mit
L5900
Linoleic Acid-Water Soluble
powder, BioReagent, suitable for cell culture
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
10 mg | Warenkorb auf Verfügbarkeit prüfen | € 49,30 |
Über diesen Artikel
NACRES:
NA.75
UNSPSC Code:
12352211
Technischer Dienst
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Unterstützung erhaltenbiological source
plant (Safflower oil)
Quality Segment
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 50 mg/mL
storage temp.
−20°C
Application
Linoleic acid-water soluble has been used as a component of fatting media for the in vitro steatosis induction of primary human hepatocytes (PHH).
Biochem/physiol Actions
Linoleic acid (LA) is an arachidonic acid precursor, which is present in vegetable oils. It enhances wound healing in diabetic rats. LA triggers tumor and apoptosis in colorectal cancer cells. The levels of LA in adipose tissue and platelets are associated with coronary artery disease. Linoleic acid binds to molecules, such as bovine serum albumin (BSA) or cyclodextrin for use in cell culture.
Other Notes
Contains approx. 30 mg of linoleic acid per gram; balance methyl-β-cyclodextrin.
Package size based on linoleic acid content.
Package size based on linoleic acid content.
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Dieser Artikel | |||
|---|---|---|---|
| description powder, BioReagent, suitable for cell culture | description powder, BioReagent, suitable for cell culture | description liquid, BioReagent, suitable for cell culture | description ≥97% (GC) |
| biological source plant (Safflower oil) | biological source synthetic (organic) | biological source plant (Safflower oil) | biological source plant oil (safflower) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) activity assay: suitable |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL, clear to slightly hazy | solubility NaOH: 1 M, ethanol: soluble | solubility methanol: 50 mg/mL, clear, yellow |
| form powder | form powder | form liquid | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Lagerklasse
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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If we dissolve the powder in distilled water, as described in one of the questions, can we aliquot this solution and freeze it at -20 or -70. If so, for how long? Or should we make a fresh solution every time?
1 answer-
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Hi there, could you confirm the validity of our stock preparation for L5900-10MG? We dissolve the full 10 mg tube in 1.43 mL distilled water, yielding a 25 millimolar stock solution.
1 answer-
Linoleic acid has a molecular weight of 280.45 g/mol. Dissolving the full 10 mg tube in 1.43 mL of distilled water yields a 25 millimolar stock solution.
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Dear, can you please clarify the composition of this product for me? In my understanding this product contains minimum 2.5% linoleic acid encapsulated in methyl-β-cyclodextrin.
1 answer-
The product contains approximately 30 mg of linoleic acid per gram; balance methyl-?-cyclodextrin.
Package size is based on linoleic acid content.More information can be found in the Data Sheet below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/218/859/e8014inf.pdfHelpful?
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How to prepare FAME from it?
1 answer-
This product is designed for use in mammalian cell culture applications as a supplement. If you are seeking a protocol for derivatization of Fatty Acids to FAMEs, please see the following: https://www.sigmaaldrich.com/technical-documents/protocol/food-and-beverage-testing-and-manufacturing/chemical-analysis-for-food-and-beverage/derivatization-of-fatty-acids-to-fames
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