The LPS is in an aqueous solution containing 0.5% triethylamine and pH adjusted to 6.5 using hydrochloric acid.
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L5543
Lipopolysaccharide aus Escherichia coli O26:B6
Ready Made solution, 1 mg/mL, 0.2 μm filtered
Synonym(e):
LPS
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
2 mL | Warenkorb auf Verfügbarkeit prüfen | € 309,00 € 231,75 |
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Unterstützung erhaltenbiological source
Escherichia coli (O26:B6)
Quality Segment
sterility
0.2 μm filtered
form
aqueous solution
concentration
1 mg/mL
color
colorless to faint yellow
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
General description
This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O26:B6. This LPS serotype has been used in studies of septic shock.
Application
Lipopolysaccharide (LPS) sind charakteristische Bestandteile der Zellwand von gramnegativen Bakterien. LPS und seine Lipid-A-Gruppe stimulieren Zellen des angeborenen Immunsystems durch den Toll-like-Rezeptor 4 (TLR4). TLR4 gehört zur Toll-like-Rezeptor-Proteinfamilie, die häufige Pathogen-assoziierte molekulare Strukturen (PAMPs) erkennt.
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Dieser Artikel | |||
|---|---|---|---|
| description Ready Made solution, 1 mg/mL, 0.2 μm filtered | description purified by trichloroacetic acid extraction | description γ-irradiated, BioXtra, suitable for cell culture | description ≥10,000 EU/mg, purified by phenol extraction |
| biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O26:B6) |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| solubility water: soluble | solubility water: soluble | solubility H2O: 5 mg/mL, slightly hazy | solubility water: soluble |
| form aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color colorless to faint yellow | color white to yellow | color - | color white to faint yellow |
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