L1882

Lektin aus Triticum vulgaris (Weizen)

agarose conjugate, saline suspension

• Synonym(e): Weizenkeim-Agglutinin
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: saline suspension

Eigenschaften

• conjugate: agarose conjugate
• Quality Segment: 200
• form: saline suspension
• extent of labeling: 5-10 mg per mL
• matrix: cross-linked 4% beaded agarose
• matrix activation: cyanogen bromide
• capacity: ≥0.1 mg/mL binding capacity (ovomucoid)
• storage temp.: 2-8°C

Beschreibung

General description

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Biochem/physiol Actions

WGA ist nicht Blutgruppen-spezifisch, hat aber eine Affinität zu N-Acetyl-β-D-Glucosaminylresten und N-Acetyl-β-D-Glucosamin-Oligomeren. WGA enthält keine proteingebundenen Kohlenhydrate.

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

Physical form

Suspension in 1,0 M NaCl mit 15 mM Natriumazid

Preparation Note

Konjugate werden aus affinitätsgereinigtem Lektin hergestellt.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• ppe: Eyeshields, Gloves