L3892

Lektin aus Triticum vulgaris (Weizen)

peroxidase conjugate, lyophilized powder

• Synonym(e): Weizenkeim-Agglutinin
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized powder
• Assay: ≥85% protein basis (Warburg-Christian)
• Biological source: Triticum vulgaris

Eigenschaften

• biological source: Triticum vulgaris
• Quality Segment: 200
• conjugate: peroxidase conjugate
• assay: ≥85% protein basis (Warburg-Christian)
• form: lyophilized powder
• potency: <40 μg/mL agglutination activity
• peroxidase activity: 50-200 units/mg protein
• composition: Protein, ~90% modified Warburg-Christian
• storage temp.: −20°C

Beschreibung

Application

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

WGA ist nicht Blutgruppen-spezifisch, hat aber eine Affinität zu N-Acetyl-β-D-Glucosaminylresten und N-Acetyl-β-D-Glucosamin-Oligomeren. WGA enthält keine proteingebundenen Kohlenhydrate.

Packaging

Packungsgrösse bezogen auf den Proteingehalt.

Physical form

Enthält Citrat-Puffer

Preparation Note

Konjugate werden aus affinitätsgereinigtem Lektin hergestellt.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan,[1] which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Other Notes

1 U bildet 1.0 mg Purpurogallin aus Pyrogallol in 20 s bei pH 6.0 und 20 °C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)