H5527

Hygromycin B -Lösung aus Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H₂O, γ-irradiated

• Synonym(e): Hygromycin b, Hygrovetine
• Empirische Formel (Hill-System): C₂₀H₃₇N₃O₁₃
• CAS-Nummer: 31282-04-9
• Molekulargewicht: 527.52
• Beilstein: 6755837
• MDL-Nummer: MFCD06795479
• UNSPSC-Code: 51102829
• PubChem Substanz-ID: 24895698
• NACRES: NA.85

Eigenschaften

• Biologische Quelle: Streptomyces hygroscopicus
• Qualitätsniveau: 200
• Sterilität: γ-irradiated
• Konzentration: ≥60% (HPLC); 45-60 mg/mL in H₂O
• Farbe: yellow-brown to orange
• Wirkungsspektrum von Antibiotika: viruses
• Wirkungsweise: protein synthesis | interferes
• Lagertemp.: 2-8°C
• SMILES String: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChIKey: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Beschreibung

Allgemeine Beschreibung

Chemical structure: aminoglycoside

Anwendung

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Biochem./physiol. Wirkung

Wirkungsweise: Das Produkt wirkt, indem es die Proteinsynthese durch die Einleitung der Fehllesung der m-RNA-Matrix in Prokaryoten hemmt, mit der Wirkung der Translationshemmung.

Antimikrobielles Spektrum: Hygromycin B wirkt gegen Bakterien, Pilze und höhere eukaryotische Zellen.

Vorsicht

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Angaben zur Herstellung

This product is a solution that is =60% (HPAE), 45-60 mg/mL in H₂O, and γ-irradiated .

Sicherheitshinweise

• Piktogramme: GHS06,GHS08,GHS05
• Signalwort: Danger
• H-Sätze: H301 + H311,H318,H330,H334
• P-Sätze: P260 - P280 - P284 - P301 + P310 - P305 + P351 + P338 - P310
• Gefahreneinstufungen: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Resp. Sens. 1
• Lagerklassenschlüssel: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter