Histones are soluble in water at a concentration of 10 mg/ml or in 0.5 N HCl at the same concentration, yielding a clear or hazy solution depending on the fraction. Histones dissolved or suspended in water should remain stable for at least 6 months when frozen in single-use aliquots.
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H5505
Histon aus Kalbsthymus
Type III-S
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 mg | Warenkorb auf Verfügbarkeit prüfen | € 69,20 |
100 mg | Warenkorb auf Verfügbarkeit prüfen | € 148,00 |
250 mg | Warenkorb auf Verfügbarkeit prüfen | € 292,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
41106305
NACRES:
NA.51
Beilstein/REAXYS Number:
1895465
MDL number:
Form:
powder
Biological source:
bovine (calf) thymus
Technischer Dienst
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Unterstützung erhaltenbiological source
bovine (calf) thymus
Quality Segment
type
Type III-S
form
powder
concentration
≥60% protein (biuret)
technique(s)
activity assay: suitable
solubility
H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
storage temp.
2-8°C
Application
Histone is suitable for use:
- as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
- in Cdk5 activity assay
- in histone H1 kinase assay
- as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
- as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells
Biochem/physiol Actions
Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.
Features and Benefits
Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.
Preparation Note
Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).
Other Notes
Characterized as mainly subgroup f1 by SDS-PAGE
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description Type III-S | description Type II-A, lyophilized powder | description ≥85% (SDS-PAGE) | description powder |
| biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine milk |
| technique(s) activity assay: suitable | technique(s) - | technique(s) membrane array: suitable | technique(s) activity assay: suitable, electrophoresis: suitable, immunocytochemistry: suitable |
| concentration ≥60% protein (biuret) | concentration - | concentration 900 μg/mL | concentration - |
| form powder | form lyophilized powder | form frozen liquid | form powder |
| solubility H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow | solubility - | solubility - | solubility H2O: insoluble (forms a cloudy suspension) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −70°C | storage temp. - |
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Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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What type of buffer or solution would be best for stabilizing histone proteins? Would ultrapure water (ddH₂O) be suitable for dissolution?
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