Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

GE28-9852-54

rProtein A GraviTrap™

Cytiva 28-9852-54, pack of 10 × 1 mL

Synonym(e):

GraviTrap

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

Verpackung

pack of 10 × 1 mL

Hersteller/Markenname

Cytiva 28-9852-54

Lagerbedingungen

(20% Ethanol)

Bettvolumen

1 mL

Kapazität

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

Lagertemp.

2-8°C

Allgemeine Beschreibung

rProtein A GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose™ Fast Flow.

Anwendung

rProtein A GraviTrap™ are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Leistungsmerkmale und Vorteile

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as rProtein A GraviTrap™ ~ 50 mg human IgG/column

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kunden haben sich ebenfalls angesehen

Slide 1 of 3

1 of 3

PD Miditrap G-25 Cytiva 28-9180-08

GE28-9180-08

PD Miditrap G-25

Column PD-10, Empty Cytiva 17-0435-01

GE17-0435-01

Column PD-10, Empty

HiTrap® Desalting Columns Cytiva 17-1408-01, pack of 5 × 5 mL

GE17-1408-01

HiTrap® Desalting Columns

Artikel

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protokolle

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.