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GE17-5056-01

HiTrap® CM Fast Flow

Cytiva 17-5056-01, pack of 5 × 1 mL

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

carboxymethyl

Beschreibung

Ion Exchanger Type (value)

Verpackung

pack of 5 × 1 mL

Hersteller/Markenname

Cytiva 17-5056-01

Parameter

42 psi

Bettgröße

7 mm × 25 mm

Bettvolumen

1 mL

Säule ID

7 mm

Matrix

6% cross-linked agarose

Partikelgröße

45-165 μm

Durchschnittlicher Durchmesser

90 μm

cleaning

2-14

working range

4-13

Eignung

suitable for bioprocess medium

Verwandte Kategorien

Allgemeine Beschreibung

HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Q, SP, DEAE adn CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

Leistungsmerkmale und Vorteile

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protokolle

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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