The source of this material is subject to change from lot to lot. However, historical information indicates the fish processors are typically located in Canada, Norway, Iceland, the FaThe source of this material is subject to change from lot to lot. However, historical information indicates the fish processors are typically located in Canada, Norway, Iceland, the Faroa Islands, and Denmark. A specific body of water is not reported.roa Islands, and Denmark. A specific body of water is not reported.
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G7041
Fish Gelatin
from cold water fish skin, powder, suitable for cell culture and generation of scaffolds.
1 of 3 reviewers received a sample product or took part in a promotion
Synonym(e):
Gelatine, Teleostean-Gelatine
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 g | Warenkorb auf Verfügbarkeit prüfen | € 43,10 |
500 g | Warenkorb auf Verfügbarkeit prüfen | € 146,00 |
Über diesen Artikel
Biological source:
fish skin
Form:
solid
Technique(s):
cell culture | mammalian: suitable
Mol wt:
60 kDa
Technischer Dienst
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Unterstützung erhaltenProduktname
Gelatine aus Haut von Kaltwasserfischen, solid
biological source
fish skin
Quality Segment
product line
BioReagent
form
solid
mol wt
60 kDa
packaging
pkg of 100 g, pkg of 500 g
technique(s)
cell culture | mammalian: suitable
surface coverage
100—200 μg/cm2
impurities
≤2% Residue on ignition (Ash), ≤20 ppm Heavy metals
solubility
acetic acid: soluble, glycerol: soluble, water: soluble
shipped in
ambient
storage temp.
room temp
General description
Gelatine aus Kaltwasserfischhaut weist niedrige Gelier- und Schmelzpunkte auf. Diese Gelatine geliert nicht bei 10 Grad Celsius und hat daher keine Gallertfestigkeit (Bloom Strength).
Application
Bei der Vorbereitung der Wasserphase wurde Gelatine aus Kaltwasserfischhaut verwendet. Es wird auch für das anhaltende Wachstum in einer statischen Kultur und bei der Flavopiridol-Behandlung und Transkript-Markierung eingesetzt.
Wird in der Immunchemie als Blockierungsmittel verwendet.
Biochem/physiol Actions
Gelatine aus Kaltwasserfischhaut kann je nach Geliereigenschaften zur Vorbereitung verschiedener Gele eingesetzt werden. Sie kann auch als Zusatzstoff bei der Surimi-Verarbeitung zur Verbesserung der funktionellen und mechanischen Eigenschaften des Gels verwendet werden.
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Dieser Artikel | |||
|---|---|---|---|
| description solid | description powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture | description gel strength ~175 g Bloom, Type A | description gel strength 300, Type A |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, electrophoresis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source fish skin | biological source Porcine skin | biological source Porcine skin | biological source Porcine skin |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solid | form powder | form powder | form powder |
| mol wt 60 kDa | mol wt 50—100 kDa | mol wt - | mol wt - |
| solubility glycerol: soluble, acetic acid: soluble, water: soluble | solubility H2O: soluble 50 mg/mL, clear to hazy, faintly yellow | solubility H2O: soluble 50 mg/mL | solubility H2O: soluble 50 mg/mL |
Lagerklasse
11 - Combustible Solids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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What waters are the fish derived from? Arctic? Icelandic? North Atlantic? If North Atlantic more specifically, Canadian, novia scotia?
1 answer-
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Could you clarify the specific type of your Cold Water Fish Skin Gelatin? Is it Type A or Type B?
1 answer-
This product is a type A gelatin extracted from acid-cured tissue.
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Hi, could you please let me know the fish species or the Amino Acid sequence of the gelatin? Thanks!
1 answer-
Unfortunately, the source species of cold-water fish for this product is not available. No sequence information is available either.
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What is the melting point of this specific fish gelatin?
1 answer-
Dry gelatin, when heated to 100 °C in the presence of air, swells and becomes soft, disintegrating to a carbonaceous mass. Solidified solutions may be reliquified at 37°C. Solutions of gelatin will undergo hydrolysis when heated above 40 °C for extended periods. Please see the link below to review additional information available in the Product Datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/548/g7041pis.pdfHelpful?
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What fish species is this gelatin product derived from? Thanks.
1 answer-
This gelatin product is produced from multiple cold water fish species. These include, but are not limited to, cod, pollock, and haddock.
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Is product G7041 acid pretreated or alkali pretreated?
1 answer-
This product is extracted from acid-cured tissue.
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Is this in solid powder form? Also, is it suitable to be used to form a hydrogel film?
1 answer-
Yes, this product is supplied in solid powder form. Gelatin, in general, can be used to form a hydrogel film, but this product has not been tested for that application.
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What is the molecular weight of this Gelatin?
What is the molecular weight of this Gelatin?
1 answer-
The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
Bloom Number Average Molecular Mass (Da)
50−125 (Low Bloom) 20,000−25,000
175−225 (Medium Bloom) 40,000−50,000
225−325 (High Bloom) 50,000−100,000Please see the link below to review the general datasheet for Gelatin:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdfHelpful?
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Could you provide me with a preparation protocol to use it as a blocking agent in ELISA for G7041 ? And at what concentration is G7041 100 g.?
1 answer-
This powdered gelatin product is not specifically tested for use as a blocking agent in Elisa. Typically, Fish Skin Gelatin is prepared in a buffer at a concentration of 1 - 5% for this application. Please see the link below to review an article that may be helpful.
https://pubmed.ncbi.nlm.nih.gov/3611792/Please see the link below to review the general Gelatin datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/548/g7041pis.pdfHelpful?
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I would like to know the primary amine and functionable carboxylic acid group contents of this product.
1 answer-
The primary amine and functionable carboxylic acid group contents of G7041 were not determined in-house.
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