G6650

Gelatine aus Rinderhaut

Type B

• CAS-Nummer: 9000-70-8
• MDL number: MFCD00081638
• EC Number: 232-554-6
• NACRES: NA.61
• UNSPSC Code: 12352202
• Form: powder
• Biological source: bovine skin

Eigenschaften

• biological source: bovine skin
• Quality Segment: 200
• type: Type B
• form: powder
• technique(s): ELISA: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable, western blot: suitable

Beschreibung

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm² or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin was used for coating cell culture to improve attachment of cells, in addition to PCR to help stabilize Taq DNA. It was used as a blocking reagent in Western blotting, ELISA, and immunohistochemistry. Gelatin can also be used as a component of media for species differentiation in bacteriology. Gelatin is a biocompatible polymer and has been used as delivery vehicle for the release of bioactive compounds and in the generation of scaffolds for engineering applications.
It was used to test keratinocyte growth factor stimulation of gelatinase (matrix metalloproteinase-9) and plasminogen activator in histiotypic epithelial cell culture. It was also used to study the changes in the nucleolar organizer regions in the tuberomammillar region after dehydration.

Gelatin has various industrial applications such as stabilizer, thickener, and texturizer in foods. It is also used in the manufacture of rubber substitutes, adhesives, cements, lithographic and printing inks, plastic compounds, artificial silk, photographic plates and films, matches, and light filters for mercury lamps. In the pharmaceutical industry, gelatin is used as a suspending agent, encapsulating agent and tablet binder. In veterinary applications it is used as a plasma expander and hemostatic sponge.

Preparation Note

This product is derived from bovine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 50−120.

Other Notes

Gelatine ist ein heterogenes Gemisch aus wasserlöslichen Proteinen mit hoher mittlerer Molekularmasse, das in Kollagen vorhanden ist. Die Proteine werden mittels Sieden der relevanten Haut, Sehnen, Ligamente, Knochen usw. in Wasser extrahiert. Gelatine vom Typ A wird aus mit Säure aufgeschlossenem Gewebe gewonnen. Gelatine vom Typ B wird aus mit Kalklauge geäschertem Gewebe gewonnen.

Disclaimer

Trockene Gelatine bleibt viele Jahre unverändert, wenn sie in einem luftdicht verschlossenen Behälter bei Raumtemperatur aufbewahrt wird. Bei einer Erhitzung auf 100 °C in Gegenwart von Luft schwillt sie auf, wird weich und zersetzt sich in eine kohlenstoffhaltige Masse, wobei Pyridinbasen und Ammonium entstehen.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)