G9136

Porcine Gelatin

from porcine skin, Type A, powder, γ-irradiated, suitable for cell culture, BioXtra

• CAS-Nummer: 9000-70-8
• MDL number: MFCD30734128
• UNSPSC Code: 12352202
• EC Number: 232-554-6
• NACRES: NA.75
• Biological source: Porcine skin
• Form: lyophilized powder
• Technique(s): cell culture | mammalian: suitable

Eigenschaften

• Produktname: Gelatine aus Schweinehaut, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• biological source: Porcine skin
• Quality Segment: 200
• sterility: γ-irradiated
• type: Type A
• product line: BioXtra
• form: lyophilized powder
• packaging: pkg of 10 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 100‑200 μg/cm²
• gel strength: ~300 g Bloom
• solubility: soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)
• shipped in: ambient
• storage temp.: room temp

Beschreibung

Application

This product is recommended for use as a cell culture substratum at 100-200 μg/cm². The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.

This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.

Other Notes

Gelatine ist ein heterogenes Gemisch aus wasserlöslichen Proteinen mit hoher mittlerer Molekularmasse, das in Kollagen vorhanden ist. Die Proteine werden mittels Sieden der relevanten Haut, Sehnen, Ligamente, Knochen usw. in Wasser extrahiert. Gelatine vom Typ A wird aus mit Säure aufgeschlossenem Gewebe gewonnen. Gelatine vom Typ B wird aus mit Kalklauge geäschertem Gewebe gewonnen.

Disclaimer

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)