G5637 is sent to the Biological Stain Commission (BSC) for certification as a biological dye. The BSC performs various tests and also tests suitability for use in staining blood films and in the Wolbach's Giemsa method for paraffin embedded tissue. There is no performance testing for the G Banding of human chromosomes. The customer would need to provide their own staining protocol or locate a published method if they intend to use G5637 for the G banding of human chromosomes.
Fortfahren mit
G5637
Giemsa-Färbung
certified by the Biological Stain Commission, powder or crystals
Synonym(e):
Giemsa Farbstoff
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 g | Warenkorb auf Verfügbarkeit prüfen | € 122,00 |
25 g | Warenkorb auf Verfügbarkeit prüfen | € 376,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C14H14ClN3S
CAS-Nummer:
Molekulargewicht:
291.80
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
Technischer Dienst
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Unterstützung erhaltenProduktname
Giemsa-Färbung, certified by the BSC
agency
certified by the BSC
Quality Segment
form
powder or crystals
technique(s)
microbe id | staining: suitable
color
dark green to very dark green
mp
300 °C (lit.)
solubility
ethanol: 1 mg/mL, blue to very deep blue
absorption
≥0.6 at 647-653 nm in methanol at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
microbiology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
General description
Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.
Application
Giemsa stain has been used for the staining of cells and blood films.It is required for the staining of azurophil granules and is an excellent stain for inclusion bodies.Its applications can be seen in hematology for blood and bone marrow specimens, bacteriology, clinical cytology specimens, histological biopsies, and tumor samples.
Zertifiziert für die Giemsa-Färbung von Blutausstrichen und für die Wolbach-Giemsa-Methode für Paraffinschnitte.
Other Notes
Nuclei will be varying shades of purple. Cytoplasmic staining will be varying shades of blue to light pink. Fine reddish to lilac granules may be present in cytoplasm of some cell types. Basophils will demonstrate dark blue black granules in the cytoplasm. Eosinophils will demonstrate bright orange granules in the cytoplasm. Red blood cells should be pink to orange
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Dieser Artikel | |||
|---|---|---|---|
| description certified by the BSC | description technical grade, used as a blood stain | description according to Giemsa | description suitable for blood stain |
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) UV/Vis spectroscopy: suitable | technique(s) microbe id | staining: suitable |
| color dark green to very dark green | color dark greenvery dark | color dark blue | color dark green |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility ethanol: 1 mg/mL, blue to very deep blue | solubility ethanol: 1 mg/mL | solubility - | solubility methanol: 10 mg/10 mL |
| form powder or crystals | form powder | form liquid | form powder |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
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Hello, is this product suitable for G banding of human chromosomes? Thank you!
1 answer-
Helpful?
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Hello, could you confirm the dye(s) present in G5367? Is it only the pictured compound (I think Azure A) without methylene blue or eosin? Thank you!
1 answer-
Giemsa stain, as defined by the Biological Stain Commission, consists of a polychrome methylene blue and eosin y mixture. When methylene blue undergoes the polychroming process, various homologs of methylene blue are expected to be present. Azure A, Azure B, Azure C, and other homologs may be present. The percentages of the various homologs can and will vary. This product is not tested to determine the identity or concentration of these homologs.
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