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G5154

Glasgow Minimum Essential Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

GMEM

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MDL number:
NACRES:
NA.75
UNSPSC Code:
12352207
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Produktname

Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

General description

For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.

Application

Glasgow Minimum Essential Medium has been used to culture:
  • Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
  • murine E14 Tg2a embryonic stem (mES) cells
  • transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins

Preparation Note

Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

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With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

form

liquid

form

powder

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

components

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

components

glucose: 4.5 g/L
phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: no

components

phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


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Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1



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