G0535

Glycopeptidase A from almonds

buffered aqueous glycerol solution, ≥0.05 unit/mL

• Synonym(e): N-Glycosidase A, N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase, PNGase A
• CAS-Nummer: 83534-39-8
• EC-Nummer: 3.5.1.52 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131222
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Konjugat: (N-linked)
• Qualitätsniveau: 200
• Form: buffered aqueous glycerol solution
• Konzentration: ≥0.05 unit/mL
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages.

Anwendung

Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.

Biochem./physiol. Wirkung

Hydrolyzes an N⁴-(acetyl-β-D-glycosaminyl)asparagine in which the N-acetyl-D-glucosamine residue may be further glycosylated, yielding a (substituted) N-acetyl-β-D-glucoaminylamine and the peptide containing an aspartic residue.

Einheitendefinition

One unit will hydrolyze 1.0 μmole of ovalbumin glycopeptide per min at pH 5.0 at 37°C.

Physikalische Form

Solution in 50% glycerol containing 50 mM citrate-phosphate buffer, pH 5.0, and BSA.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): No data available
• Flammpunkt (°C): No data available