The volume of the container is 2 mL. Reconstitution of this product should be performed using 0.2 µm filtered 20 mM Tris, pH 7.0, to prepare a stock solution of >= 25 ug/mL. This stock solution may be diluted immediately before use to achieve the final working concentration. Additional filtration is not recommended, as it may result in product loss due to adsorption onto the filter membrane.
F0291
Fibroblast Growth Factor-Basic, human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(e):
Fibroblasten-Wachstumsfaktor, basisch human, FGF2, bFGF
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Über diesen Artikel
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
16.0 kDa
Impurities:
≤1.00 EU/μg
Fortfahren mit
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenProduktname
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μg, pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg
color
white to faint yellow cast
solubility
water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... FGF2 (2247)
Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| biological source human | biological source human | biological source bovine pituitary glands | biological source human |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable |
| assay ≥97% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ~90% (SDS-PAGE and N-terminal analysis) | assay >95% (SDS-PAGE) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form - |
General description
Basischer Fibroblasten-Wachstumsfaktor (FGFb) ist in Basalmembranen und in der subendothelialen extrazellullären Matrix zu finden. FGFb wird früh in der embryonalen Entwicklung induziert, bei der er für die Pluripotenz und Selbsterneuerung embryonaler Stammzellen (ES-Zellen) und liniendefinierter Stammzellen erforderlich (jedoch nicht ausreichend) ist; wobei er Proliferation unterstützt und Differenzierung hemmt. Die Selbsterneuerung von hES-Zellen hängt von der Aktivierung des Activin/Nodal/Smad2,3-Wegs und Unterdrückung des BMP/GDF/Smad5-Wegs der TGFβ-Signalgebung ab. FGFb ist ein Kompetenzfaktor für die Nodal-Unterstützung der Pluripotenz. Activin-A ist zur Erhaltung der Stammzelleneigenschaften erforderlich und ausreichend. Es induziert die Expression sowohl von Nodal als auch FGFb in humanen ES-Zellen.
Application
Physical form
Lyophilisiert aus 0,2 μm filtrierter Lösung in 20 mM Tris und 1 M NaCl, pH 7,0, enthält 50 μg Rinderserumalbumin pro 1 μg FGFb.
Analysis Note
Die Bioaktivität von FGFb wurde in einem fluorometrischen Assay unter Einsatz des Redox-empfindlichen Farbstoffs Resazurin gemessen.
Ähnliches Produkt
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Lei Xiao et al.
Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)
Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an
R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
Brad Davidson et al.
Genes & development, 20(19), 2728-2738 (2006-10-04)
Comprehensive gene networks in Ciona intestinalis embryos provide a foundation for characterizing complex developmental processes, such as the initial phases of chordate heart development. The basic helix-loop-helix regulatory gene Ci-Mesp is required for activation of cardiac transcription factors. Evidence is
Artikel
Fibroblast growth factors in cell culture and various growth factors for your research
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
Protokolle
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
-
What is the volume of the vial and how do I reconstitute the powder?
1 answer-
Helpful?
-
-
Hey! I am using this product to prepare a stem cell media. I know the concentration is 0.025mg but it has two data sheets, one that its soluble in water (0.250ml) and other that says to dissolve in 20mM Tris Base pH 7.0. How many volume should I add?
1 answer-
The solubility of this product is tested in water. It is lyophilized from a solution containing 20 mM Tris and 1M NaCl with 50 ug BSA as a stabilizer. The product data sheet recommends reconstituting in the 20 mM Tris for optimum stability and long term storage.
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung