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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-803-9
MDL number:
Specific activity:
10-25 units/mg solid
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Dieser Artikel | |||
|---|---|---|---|
| specific activity 10-25 units/mg solid | specific activity 100-250 units/mg protein | specific activity 400-800 units/mg protein | specific activity - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
Application
Dextranase from Penicillium sp. has been used in a study to assess a new method of synthesizing biopolymeric affinity ligands. Dextranase from Penicillium sp. Has also been used in a study to investigate the cloning and sequencing of a dextranase-encoding cDNA from Penicillium minioluteum.
Biochem/physiol Actions
An endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.[3]
Analysis Note
Crude
Other Notes
One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Polysaccharide synthesis by Phanerochaete chrysosporium during degradation of kraft lignin
Leisola M, et al.
Europ. J. Appl. Microbiol. Biotechnol., 15(3), 180-184 (1982)
Synthesis of new enzymatically degradable thermo-responsive nanogels.
Aguirre, G., Ramos, J., & Forcada, J
Soft Matter, 9(1), 261-270 (2013)
H Roca et al.
Yeast (Chichester, England), 12(12), 1187-1200 (1996-09-30)
The DEX gene encoding an extracellular dextranase was isolated from the genomic DNA library of Penicillium minioluteum by hybridization using the dextranase cDNA as a probe. Comparison of the gene and cDNA sequences revealed that the DEX gene does not
G S Chaga et al.
Biotechnology and applied biochemistry, 26 ( Pt 1), 7-14 (1997-08-01)
(1) A new concept for producing soluble polymeric affinity ligands is proposed and exemplified. By solid-phase synthesis, an insoluble hydrophilic polymer is converted into an affinity gel. The gel is hydrolytically degraded to water-soluble affinity polymeric ligands which are recovered
B Garcia et al.
FEMS microbiology letters, 143(2-3), 175-183 (1996-10-01)
A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of selected clones
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