Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.
Alle Fotos(1)
Wichtige Dokumente
D0426
DAB with Metal Enhancer
SIGMAFAST™, peroxidase substrate, tablet
Synonym(e):
3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
5 SETS
€ 48,70
50 SETS
€ 214,00
€ 48,70
Check Cart for Availability
Alle Fotos(1)
Größe auswählen
Ansicht ändern
5 SETS
€ 48,70
50 SETS
€ 214,00
About This Item
UNSPSC-Code:
12352204
NACRES:
NA.83
€ 48,70
Check Cart for Availability
Empfohlene Produkte
Produktbezeichnung
SIGMAFAST™ DAB with Metal Enhancer, tablet
Form
tablet
Löslichkeit
water: 1 tablet/5 mL
Lagertemp.
−20°C
Verwandte Kategorien
Allgemeine Beschreibung
SIGMAFAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Anwendung
SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry,[1] dorsal root ganglion (DRG) neurons[2] and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.[3]
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
Rekonstituierung
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.
Rechtliche Hinweise
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Signalwort
Danger
Gefahreneinstufungen
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Developing rat lung has a sided pacemaker region for morphogenesis-related airway peristalsis
Jesudason EC, et al.
American Journal of Respiratory Cell and Molecular Biology, 32(2), 118-127 (2005)
Cdk5 inhibitor roscovitine alleviates neuropathic pain in the dorsal root ganglia by downregulating N-methyl-D-aspartate receptor subunit 2A
Yang L, et al.
Neurological Sciences, 35(9), 1365-1371 (2014)
Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 176, 124-135 (2017-10-11)
In most of the avian species, daylength or photoperiod is the main environmental factor regulating reproduction. During their annual gonadal cycle, birds once sensitive to short or long day effect develop refractoriness to the same daylength and gonad develop or
Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture
Cheng M, et al.
Tissue Engineering: Part A, 16(5), 1479-1489 (2010)
Saba Shahin et al.
Toxicological sciences : an official journal of the Society of Toxicology, 161(2), 349-374 (2017-10-27)
Microwave (MW) radiation induced oxidative stress reduces dendritic arborization, spine density and number of hippocampal pyramidal neurons and hence, impair learning and spatial memory through p53-dependent/independent apoptosis of hippocampal neuronal and nonneuronal cells. However, the mechanisms responsible for MW radiation
Artikel
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protokolle
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
-
How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?
1 answer-
Helpful?
-
-
What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?
1 answer-
This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?
1 answer-
The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
