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Über diesen Artikel
NACRES:
NA.51
UNSPSC Code:
41105500
Form:
solution (in 10 mM Tris, 1 mM EDTA)
Storage temp.:
−20°C
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenform
solution (in 10 mM Tris, 1 mM EDTA)
packaging
pkg of 2 vials
pH range
7.5-8.5
shipped in
ambient
storage temp.
−20°C
General description
EUR-2019-nCoV Assays are for qPCR (5′ nuclease assay) detection of SARS-CoV-2.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
Features and Benefits
The forward and reverse primers in each tube come with the following features:
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- No modifications
- Desalt purified
- 8 nmol each E_Sarbeco & 10 nmol each Modified RdRp
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- 6-FAM™ reporter
- BXQ (Block All Quencher—SigmaAldrich.com/bxq)
- HPLC purified
- 4 nmol E_Sarbeco & 5 nmol Modified RdRp
Other Notes
The product includes 2 individual tubes of 20X primer and probe pools for quantification of SARS-CoV-2 genes. Assays included are E_Sarbeco and Modified RdRp (formerly Discrimatory) genes.
The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM (E_Sarbeco); and, 10, 10, and 5 μM (Modified RdRp), respectively.
E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM (E_Sarbeco); and, 10, 10, and 5 μM (Modified RdRp), respectively.
E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
Legal Information
FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
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Dieser Artikel | |||
|---|---|---|---|
| description - | description - | description - | description EURM Reference Material |
| form solution (in 10 mM Tris, 1 mM EDTA) | form solution (in 10 mM Tris, 1 mM EDTA) | form solution (in 10 mM Tris, 1 mM EDTA) | form - |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of 2 vials | packaging pkg of 1 vial | packaging pkg of 1 vial | packaging - |
| pH range 7.5-8.5 | pH range 7.5-8.5 | pH range 7.5-8.5 | pH range - |
Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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