CHA00011B

EUR-2019-nCoV RdRp D for detection of SARS-CoV-2 RNA

• NACRES: NA.51
• UNSPSC Code: 41105500
• Form: solution (in 10 mM Tris, 1 mM EDTA)
• Storage temp.: −20°C

Eigenschaften

• form: solution (in 10 mM Tris, 1 mM EDTA)
• packaging: pkg of 1 vial
• pH range: 7.5-8.5
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

EUR-2019-nCoV Modified RdRp (formerly Discrimatory) Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2.

The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.

For additional details, please visit SigmaAldrich.com/ncov.

Features and Benefits

The forward and reverse primers in each tube come with the following features:

• DNA bases
• No modifications
• Desalt purified
• 10 nmol each

The Dual-Labeled Probe in each tube comes with the following features:

• DNA bases
• 6-FAM^™ reporter
• BXQ (Block All Quencher—SigmaAldrich.com/bxq)
• HPLC purified
• 5nmol

Other Notes

The product includes 1 individual tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene. Assay included is Modified RdRp.

The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 5 μM, respectively.

Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC

Legal Information

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable