CE0050
CelLytic™ MEM Protein Extraction Kit
Synonym(e):
Membrane protein extraction kit
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€ 513,00
€ 513,00
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NACRES:
NA.56
UNSPSC Code:
41116134
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) protein extraction: suitable | technique(s) protein extraction: suitable | technique(s) - | technique(s) - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. room temp | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.[1]
Application
CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes[2] and to extract membrane proteins for western blotting.[3][4]
Sufficient reagents supplied for 80 tests.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL
Lagerklasse
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 2
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2
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The Journal of clinical endocrinology and metabolism, 102(4), 1375-1386 (2017-03-23)
TMEM165 deficiency is a severe multisystem disease that manifests with metabolic, endocrine, and skeletal involvement. It leads to one type of congenital disorders of glycosylation (CDG), a rapidly growing group of inherited diseases in which the glycosylation process is altered.
Elisabeth P Carpenter et al.
Current opinion in structural biology, 18(5), 581-586 (2008-08-05)
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to
Sushmitha Vijaya Kumar et al.
Scientific reports, 10(1), 14985-14985 (2020-09-13)
Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted
Hiroaki Tateno et al.
Glycobiology, 26(12), 1328-1337 (2016-04-03)
Human somatic stem cells such as human mesenchymal stem cells (hMSCs) are considered attractive cell sources for stem cell-based therapy. However, quality control issues have been raised concerning their safety and efficacy. Here we used lectin microarray technology to identify
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