C1990

CelLytic^™ Express

• Synonym(e): Bacterial cell lysis reagent
• NACRES: NA.56
• UNSPSC Code: 41116134

Eigenschaften

• form: powder
• Quality Segment: 200
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

CelLytic^™ Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic^™ Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris and is immediately ready for affinity purification. CelLytic^™ Express unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

Application

CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris, and is immediately ready for affinity purification. CelLytic Express′ unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

CelLytic^™ Express has been used as a component in lysis buffer for protein purification.[1]
CelLytic Express is optimized for the lysis of E. coli strain BL21. However, it works well with other commons strains such as JM109. CelLytic Express may also be used on other similar bacterial cells. CelLytic Express has been tested with BL21 E. coli cells expressing histidine-tagged and FLAG fusion proteins. It should be compatib with affinity purification of other fusion proteins.

Features and Benefits

• Efficient and non-denaturing in-culture protein extraction
• Save time by eliminating cell harvest and clarification steps
• Preserve biological activity with fewer manipulations
• Compatible with affinity resins and magnetic beads
• Ideal for Production Scale Extraction protocols

Preparation Note

CelLytic^™ Express is supplied ready-to-use. However, additional reagents may be used depending on the nature of the protein to be isolated. These include salts, protease inhibitors, and reducing agents such as dithiothreitol or 2-mercaptoethanol. The additional reagents should be added into the bacterial culture after dissolving the CelLytic^™ Express, and not by adding them to the dry powder.

This product ships on wet ice and storage at –20°C is recommended. CelLytic^™ Express, as supplied, is stable for at least two years when stored properly.

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3