JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: JVM-2, 96090515
• Biologische Quelle: human unknown/unspecified
• Wachstumsmodus: Suspension
• Karyotyp: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

Beschreibung der Zelllinie

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

Anwendung

Ig expression of neoplastic cells

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

Nährmedium

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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