A10-85

93040117, rat nerve, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: A10-85, 93040117
• Biologische Quelle: rat nerve
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 42
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat glioma

Beschreibung der Zelllinie

The glioma cell line A10-85 is a subclone of A10 cells which originally emerged from the parent line IA2 by reducing the serum concentration from 15% to 10%. This parent line IA2 was derived from a rat brain glioma induced transplacentally by ethylnitroso urea (inbred BDIxrats). A10-85 cells form processes in response to cAMP. GAPDH can be induced by hydrocortisone succinate and good growth was obtained in semi-solid medium. Injection of cells into syngeneric animals resulted in the formation of malignant astrocytic tumours after short latent periods.

Anwendung

Experimental tumour models, carcinogenesis studies

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:50 to 1:100 seeding at 5x10,000 to 1x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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