V79-4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93010723, hamster lung, Fibroblast

• UNSPSC Code: 41106514
• Biological source: hamster lung
• Growth mode: Adherent
• Karyotype: 2n = 22
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: hamster lung
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 93010723-DNA-5UG, pkg of vial of cells 93010723-1VL
• growth mode: Adherent
• karyotype: 2n = 22
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Mutagenesis and mammalian cell genetic studies

Biochem/physiol Actions

Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).

Hamster Chinese lung

Preparation Note

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm²; 5% CO₂; 37°C using 0.25% trypsin/EDTA.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.