V79-HG04

87020406, hamster lung, Fibroblast-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: V79-HG04, 87020406
• Biologische Quelle: hamster lung
• Wachstumsmodus: Adherent
• Karyotyp: Aneuploid
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Hamster Chinese embyro lung fibroblast transformed

Beschreibung der Zelllinie

A spontaneously transformed cell line of a primary culture derived from embryonic lung fibroblasts of a Chinese hamster. Thioguanine resistant, HAT sensitive, high cloning efficiency in soft agar and used for HPRT- genotyping selection in HAT.

Anwendung

Cell fusion studies

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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