CCRF-SB Human Blood, ECACC 89090405

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human blood
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: 2n = 46
• Morphology: Lymphoblastoid
• Manufacturer/tradename: ECACC

Eigenschaften

• Produktname: CCRF-SB, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: human blood
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89090405-DNA-5UG, pkg of vial of cells 89090405-1VL
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

HLA profile studies

Biochem/physiol Actions

A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.

Human Caucasian acute lymphoblastic leukaemia

Preparation Note

Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO₂; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.