CCRF-CEM

85112105, human blood, Lymphoblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: CCRF-CEM, 85112105
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian acute lymphoblastic leukaemia

Beschreibung der Zelllinie

A T lymphoblastoid line obtained from the peripheral blood of a 4 year old Caucasian female with acute lymphoblastoid leukaemia. These may be grown to a high density in a spinner type suspension culture. Cells are reported to be free of virus particles as determined by electron microscopy.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19

Nährmedium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 2-3x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.

Sonstige Hinweise

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