HT 1376

87032402, human bladder, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: HT 1376, 87032402
• Biologische Quelle: human bladder
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, hypertetrapoid
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian bladder carcinoma

Beschreibung der Zelllinie

Derived from a 58 year old caucasian female with a grade 3 transitional carcinoma of the bladder who had not received chemo or radiation therapy. The cells grow in soft agar and are tumorigenic in mice and hamsters. The patient donor had not received chemotherapy or radiation treatment.

Anwendung

Tumourigenicity studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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