RPMI 7666

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human blood
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: 2n = 46
• Morphology: Lymphoblast
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human blood
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 87012703-DNA-5UG, pkg of vial of cells 87012703-1VL
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblast
• products: Immunoglobulin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Tumourigenicity studies

Biochem/physiol Actions

Human Caucasian lymphoblast

Initiated from the leukocytes obtained from a 32 year old Caucasian male having no known malignancy. EBV particles are present. The cells produce immunoglobulins in vitro.

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.