GH3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012603, rat brain, Epithelial

• UNSPSC Code: 41106514
• Biological source: rat brain
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Hyperdiploid, modal no. 60
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: rat brain
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 87012603-DNA-5UG, pkg of vial of cells 87012603-1VL
• growth mode: Adherent
• karyotype: Hyperdiploid, modal no. 60
• morphology: Epithelial
• products: Growth hormone, prolactin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Hormone studies

Biochem/physiol Actions

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

Rat Wistar-Furth pituitary tumour

Preparation Note

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.