CHO Cell Line from Chinese hamster ovary

85050302, hamster ovary, Epithelial

• Synonym(e): CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
• UNSPSC-Code: 41106514

Eigenschaften

• product name: CHO Cell Line from Chinese hamster ovary, 85050302, from hamster ovary
• Biologische Quelle: hamster ovary
• Verfügbarkeit: not available in Japan
• Wachstumsmodus: Adherent
• Karyotyp: Hypodiploid, modal no. 20
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Hamster Chinese ovary

Beschreibung der Zelllinie

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

Anwendung

CHO Cell Line from Chinese hamster ovary has been used:

• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

Nährmedium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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