RCC4plusVHL

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 3112703, human kidney, Epithelial

• Synonym(e): RCC4 VHL(+) Cells, RCC4-pVHL Cells, RCC4/VHL Cells
• UNSPSC Code: 41106514
• Biological source: human kidney
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human kidney
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 03112703-DNA-5UG, pkg of vial of cells 03112703-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Study of tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. Cell responses to hypoxia.

Biochem/physiol Actions

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL. The plasmid pcDNA3-VHL confers neomycin resistance and encodes the von Hippel-Lindau (VHL) tumour suppressor gene product pVHL. The original renal carcinoma cell line RCC4 is VHL-deficient. The RCC4 plus vector alone cell line (Sigma catalogue no. 03112702) can serve as a negative control cell line to study the effect of pVHL expression from pcDNA3-VHL. These cells are less tumourigenic than corresponding cell lines lacking pVHL function.

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

Preparation Note

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.