Pericyte isolation generally starts with enzymatic digestion of tissue with Trypsin EDTA, usually followed by the isolation of microvessel fragments via successive filtration steps. Pure pericyte cultures can be obtained through either positive or negative immuno-selection via the use of magnetic beads or flow cytometry using the antibody markers above.
Fortfahren mit
C-12981
Human Pericytes from Placenta (hPC-PL)
500,000 proliferating cells
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 ea | Warenkorb auf Verfügbarkeit prüfen | € 1.570,00 |
Über diesen Artikel
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
placenta
Morphology:
( pericytes)
Technischer Dienst
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Unterstützung erhaltenbiological source
placenta
packaging
pkg of 500,000 cells
morphology
( pericytes)
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
room temp
General description
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Application
Human Pericytes are isolated from human placenta tissue and provided in a proliferating format. Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas. To date, pericytes have demonstrated the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytes (macrophages).
Biochem/physiol Actions
Placenta
Preparation Note
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Analysis Note
Rigid quality control tests are performed for each lot of Pericytes. The cells are tested for morphology, adherence rate, and viability. Furthermore, they are characterized by flow cytometric analysis for a comprehensive panel ofmarkers, e.g. CD31, CD34, CD105, and CD146. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
Recommended Plating Density: 4000 cells per cm2Tested markers: CD146+, CD105+, CD31-, CD34-
Recommended Primary Cell Culture Media:Link
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
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Dieser Artikel | |||
|---|---|---|---|
| description 500,000 proliferating cells | description 500,000 cryopreserved cells | description 1 million cells per pellet | description 500,000 cryopreserved cells |
| morphology ( pericytes) | morphology ( pericytes) | morphology (pericyte) | morphology (fibroblast) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| biological source placenta | biological source human placenta | biological source human placenta | biological source human lung |
| shipped in ambient | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. room temp | storage temp. −196°C | storage temp. −20°C | storage temp. −196°C |
| packaging pkg of 500,000 cells | packaging pkg of 500,000 cells | packaging pkg of 1,000,000 cells | packaging pkg of 500,000 cells |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How are pericytes isolated?
1 answer-
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How long do pericytes grow in culture?
1 answer-
PromoCell’s human pericytes derived from microvessels of the human placenta are cryopreserved at the end of secondary culture (P1) and after thawing are considered P2. The number of populations doublings is not determined for each individual cell lot, however they are guarenteed to be grown for at least 15 population doublings.
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What markers are used to characterize pericytes?
1 answer-
Pericytes are positive for CD146, PDGFRβ, αSMA, CD13, NG2, Desmin, and negative for CD31, CD34, CD45, and CD56.
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