PromoCell’s human pericytes derived from microvessels of the human placenta are cryopreserved at the end of secondary culture (P1) and after thawing are considered P2. The number of populations doublings is not determined for each individual cell lot, however they are guarenteed to be grown for at least 15 population doublings.
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C-14095
Human Pericytes from Placenta (hPC-PL)
1 million cells per pellet
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Über diesen Artikel
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human placenta
Morphology:
(pericyte)
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenbiological source
human placenta
packaging
pkg of 1,000,000 cells
morphology
(pericyte)
application(s)
cell analysis (DNA, RNA, and protein)
shipped in
dry ice
storage temp.
−20°C
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.
Application
Human Pericytes are isolated from human placenta tissue. Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas. To date, pericytes have demonstrated the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytes (macrophages).
Biochem/physiol Actions
Placenta
Physical form
Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.
Preparation Note
Click here for more information.
Analysis Note
Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.
Legal Information
RNAlater is a registered trademark of Thermo Fisher Scientific, Inc.
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Dieser Artikel | |||
|---|---|---|---|
| description 1 million cells per pellet | description 500,000 cryopreserved cells | description 500,000 cryopreserved cells | description 500,000 proliferating cells |
| biological source human placenta | biological source human placenta | biological source human lung | biological source placenta |
| morphology (pericyte) | morphology ( pericytes) | morphology (fibroblast) | morphology ( pericytes) |
| storage temp. −20°C | storage temp. −196°C | storage temp. −196°C | storage temp. room temp |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in ambient |
| packaging pkg of 1,000,000 cells | packaging pkg of 500,000 cells | packaging pkg of 500,000 cells | packaging pkg of 500,000 cells |
| application(s) cell analysis (DNA, RNA, and protein) | application(s) - | application(s) - | application(s) - |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
7783-20-2
CAS No.
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How long do pericytes grow in culture?
1 answer-
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How are pericytes isolated?
1 answer-
Pericyte isolation generally starts with enzymatic digestion of tissue with Trypsin EDTA, usually followed by the isolation of microvessel fragments via successive filtration steps. Pure pericyte cultures can be obtained through either positive or negative immuno-selection via the use of magnetic beads or flow cytometry using the antibody markers above.
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What markers are used to characterize pericytes?
1 answer-
Pericytes are positive for CD146, PDGFRβ, αSMA, CD13, NG2, Desmin, and negative for CD31, CD34, CD45, and CD56.
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