C0733

Cyclooxygenase 1 from sheep

glycerol solution, ≥1500 units/mg protein

• Synonym(e): COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase
• EC-Nummer: 1.14.99.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD02098124
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Form: glycerol solution
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥1500 units/mg protein
• Mol-Gew.: dimer subunit mol wt 70 kDa
• UniProt-Hinterlegungsnummer: O78749
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: sheep ... COX1(808251)

Beschreibung

Allgemeine Beschreibung

Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain.[1] Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.[2]

Anwendung

Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples[3] and in prostaglandin synthase activity assay.[4]

Biochem./physiol. Wirkung

COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H₂ (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent K_M of 8.3 μM for arachidonic acid.

Einheitendefinition

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

Physikalische Form

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN^® 20, 300 μM diethyldithiocarbamate and 10% glycerol.

Rechtliche Hinweise

TWEEN is a registered trademark of Croda International PLC

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable